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pink flamindo  (Addgene inc)


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    Structured Review

    Addgene inc pink flamindo
    Pink Flamindo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pink+flamindo/Pink+Flamindo+(Plasmid+%23102356)/pm41830514-208-0-6
    Average 93 stars, based on 30 article reviews
    pink flamindo - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Monoclonal Line of Cells Co-Expressing Genetically Encoded Sensors for cAMP and Ca2+
    Article Snippet: A variety of surface receptors and intracellular signaling systems are involved in cell-to-cell communication and paracrine/autocrine regulation of cellular functions.. Being most numerous, the family of G-protein coupled receptors (GPCRs) is involved in the regulation of almost all physiological processes due to coupling to multiple and diverse intracellular signaling cascades.. Among them, the ubiquitous contributors are the adenylate cyclase cascade, which controls the intracellular cAMP level, and the phosphoinositide cascade determining many aspects of intracellular Ca2+ signaling.

    Transfection:

    Article Title: Monoclonal Line of Cells Co-Expressing Genetically Encoded Sensors for cAMP and Ca2+
    Article Snippet: A variety of surface receptors and intracellular signaling systems are involved in cell-to-cell communication and paracrine/autocrine regulation of cellular functions.. Being most numerous, the family of G-protein coupled receptors (GPCRs) is involved in the regulation of almost all physiological processes due to coupling to multiple and diverse intracellular signaling cascades.. Among them, the ubiquitous contributors are the adenylate cyclase cascade, which controls the intracellular cAMP level, and the phosphoinositide cascade determining many aspects of intracellular Ca2+ signaling.

    Plasmid Preparation:

    Article Title: Monoclonal Line of Cells Co-Expressing Genetically Encoded Sensors for cAMP and Ca2+
    Article Snippet: A variety of surface receptors and intracellular signaling systems are involved in cell-to-cell communication and paracrine/autocrine regulation of cellular functions.. Being most numerous, the family of G-protein coupled receptors (GPCRs) is involved in the regulation of almost all physiological processes due to coupling to multiple and diverse intracellular signaling cascades.. Among them, the ubiquitous contributors are the adenylate cyclase cascade, which controls the intracellular cAMP level, and the phosphoinositide cascade determining many aspects of intracellular Ca2+ signaling.

    Article Title: High-throughput screening for Cushing’s disease: therapeutic potential of thiostrepton via cell cycle regulation
    Article Snippet: .. Pink Flamindo was a gift from Tetsuya Kitaguchi (Addgene plasmid #102356) ( ). .. A large amount of these plasmid vectors was prepared using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany).

    Article Title: PI3 kinase-unrelated effects of LY294002 and LY303511 on serotonin-induced Ca 2+ and cAMP signaling
    Article Snippet: .. Pink Flamindo was a gift from Tetsuya Kitaguchi (Addgene plasmid # 102356; http://n2t.net/addgene:102356 ; RRID:Addgene_102356) [ ] and PH(Akt)-Venus was a gift from Narasimhan Gautam (Addgene plasmid #85223; http://n2t.net/addgene:85223 ; RRID:Addgene_85223) [ ]. ..

    In Vivo:

    Article Title: Optogenetic Control of Dopamine Receptor 2 Reveals a Novel Aspect of Dopaminergic Neurotransmission in Motor Function
    Article Snippet: .. For in vivo application, OptoDRD2-EYFP was subcloned into a pAAV-Ef1a-DIO vector, and viruses were produced in the Korea Institute of Science and Technology (KIST) virus facility. pink-Flamindo was obtained from Addgene, and pGlo-22F was obtained from Promega. .. The human embryonic kidney 293A (HEK293A) cell line was maintained in DMEM supplemented with 10% fetal bovine serum (Hyclone), 1 unit per ml penicillin, 100 μg per ml streptomycin, and 100 μM MEM nonessential amino acid solution (Gibco).

    Article Title: Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function
    Article Snippet: Research Articles | Systems/Circuits Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function https://doi.org/10.1523/JNEUROSCI.1473-24.2024 Received: 4 August 2024 Revised: 9 November 2024 Accepted: 11 November 2024 Copyright © 2024 Kim et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data.

    Produced:

    Article Title: Optogenetic Control of Dopamine Receptor 2 Reveals a Novel Aspect of Dopaminergic Neurotransmission in Motor Function
    Article Snippet: .. For in vivo application, OptoDRD2-EYFP was subcloned into a pAAV-Ef1a-DIO vector, and viruses were produced in the Korea Institute of Science and Technology (KIST) virus facility. pink-Flamindo was obtained from Addgene, and pGlo-22F was obtained from Promega. .. The human embryonic kidney 293A (HEK293A) cell line was maintained in DMEM supplemented with 10% fetal bovine serum (Hyclone), 1 unit per ml penicillin, 100 μg per ml streptomycin, and 100 μM MEM nonessential amino acid solution (Gibco).

    Article Title: Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function
    Article Snippet: Research Articles | Systems/Circuits Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function https://doi.org/10.1523/JNEUROSCI.1473-24.2024 Received: 4 August 2024 Revised: 9 November 2024 Accepted: 11 November 2024 Copyright © 2024 Kim et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data.

    Virus:

    Article Title: Optogenetic Control of Dopamine Receptor 2 Reveals a Novel Aspect of Dopaminergic Neurotransmission in Motor Function
    Article Snippet: .. For in vivo application, OptoDRD2-EYFP was subcloned into a pAAV-Ef1a-DIO vector, and viruses were produced in the Korea Institute of Science and Technology (KIST) virus facility. pink-Flamindo was obtained from Addgene, and pGlo-22F was obtained from Promega. .. The human embryonic kidney 293A (HEK293A) cell line was maintained in DMEM supplemented with 10% fetal bovine serum (Hyclone), 1 unit per ml penicillin, 100 μg per ml streptomycin, and 100 μM MEM nonessential amino acid solution (Gibco).

    Article Title: Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function
    Article Snippet: Research Articles | Systems/Circuits Optogenetic control of dopamine receptor 2 reveals a novel aspect of dopaminergic neurotransmission in motor function https://doi.org/10.1523/JNEUROSCI.1473-24.2024 Received: 4 August 2024 Revised: 9 November 2024 Accepted: 11 November 2024 Copyright © 2024 Kim et al.. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.. This Early Release article has been peer reviewed and accepted, but has not been through the composition and copyediting processes.The final version may differ slightly in style or formatting and will contain links to any extended data.

    Cloning:

    Article Title: Spatiotemporal dynamics of β-arrestin-mediated Src activation in 5-HT7 receptor signaling pathway.
    Article Snippet: .. Pink Flamindo (#102356) was achieved from Addgene. pcDNA5HA-Flag-5-HT7R-mScarlet, pRK5-EGFP-FYVE, pEGFPC-h-Rab7a-c-myc, pmTagBFP2-C-h-Rab7a-c-myc, and pCMV-ARRB2-mYFP were prepared using general cloning methods. .. For example, the PCR products of human 5-HT7R and mScarlet were achieved from the PRESTOTANGO kit (#1000000068) and mScarlet-C1 (#85042), respectively, and fused to construct pcDNA5-HA-Flag-5HT7R-mScarlet by the In-Fusion technique (ClonTech, Mountain view, CA, USA).



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